rabbit anti atf3  (Novus Biologicals)

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Expression and diagnostic significance of ATF3 in pan-cancer. A ATF3 expression in normal and cancer samples analyzed with the GTEx and TCGA datasets. B Receiver operating characteristic (ROC) analysis evaluated the diagnostic performance of ATF3 across cancer types. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Expressing, Diagnostic Assay

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Prognostic evaluation of ATF3 in pan-cancer. A – D Univariate Cox regression analyses of ATF3 in different tumors for disease-free interval (DFI) ( A ), disease-specific survival (DSS) ( B ), overall survival (OS) ( C ) and progression-free interval (PFI) ( D ). E – H Patients were stratified by the median ATF3 expression; Kaplan-Meier curves compared the impact of high vs. low ATF3 expression on DFI ( E ), DSS ( F ), OS ( G ) and PFI ( H ) in each cancer type. HR, hazard ratio; CI, confidence interval

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Expressing

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Mutation landscape and mutation-load analysis of ATF3 in pan-cancer. A Frequencies of distinct ATF3 mutation types across cancers. B Analysis showing that missense mutations (green) were the predominant ATF3 alteration. C Radar plot depicting the correlation between ATF3 expression and tumor mutation burden (TMB). D Radar plot depicting the correlation between ATF3 expression and microsatellite instability (MSI). *, P < 0.05; **, P < 0.01

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Mutagenesis, Expressing

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Relationship between ATF3 expression and immunity/differentially methylated probes-based stemness index (DMPsi). A ESTIMATE analysis of the association between ATF3 expression and ImmuneScore, StromalScore, and EstimateScore. B – F Correlations of ATF3 expression with immune-checkpoint genes ( B ), chemokines (C), immune inhibitors (D), immune stimulators (E) and chemokines receptors (F). G Correlations between ATF3 and mismatch-repair and DNA-methyltransferase genes. H Correlation between ATF3 and DMPsi. *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Expressing, Methylation

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: ATF3 was down-regulated in kidney clear cell renal cell carcinoma (KIRC) and associated with favorable prognosis. A – C ATF3 expression in KIRC cell lines determined by real-time quantitative polymerase chain reaction (RT-qPCR) ( A ), Western blot ( B ) and immunofluorescence ( C ). D Kaplan-Meier survival curves comparing high vs. low ATF3 expression for DFI, DSS, OS and PFI in KIRC. E Association between ATF3 and clinical-feature subgroups in KIRC. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Immunofluorescence

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Enrichment and immune-infiltration analyses of ATF3. A Volcano plot of differentially expressed genes (DEGs) between high and low ATF3 groups. B Heat-map of the DEGs. C Gene Ontology (GO) enrichment of the DEGs. BP, Biological Process; CC, Cellular Component; MF, Molecular Function. D Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of the DEGs. E Gene set enrichment analysis (GSEA) of ATF3. F Stacked bar chart of immune-cell scores in high and low ATF3 groups. G Box plot showing differences in immune-cell infiltration between the two groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques:

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Prediction of ATF3-targeted drugs. A Drug-sensitivity analysis between high and low ATF3 groups (top 10 agents). B ATF3-drug network. C Molecular-docking affinity between progesterone and ATF3

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques:

Journal: Discover Oncology

Article Title: Comprehensive analysis of ATF3 as a diagnostic and prognostic biomarker from pan-cancer to clear cell renal cell carcinoma

doi: 10.1007/s12672-026-05113-x

Figure Lengend Snippet: Single-cell RNA sequencing (scRNA-seq) analysis of ATF3 in KIRC. A Uniform Manifold Approximation and Projection (UMAP) showing cell annotations. B Proportions of annotated cells in KIRC vs. control. C ATF3 expression across cell types in KIRC vs. control. D Pseudotime trajectory of endothelial-afferent/efferent arterioles/descending vasa recta (AEAs/DVR) divided into three stages. E Distribution of KIRC and control samples along the AEAs/DVR differentiation trajectory. F ATF3 expression during AEAs/DVR differentiation. G Cell-cell communication analysis. *, P < 0.05; **, P < 0.01; ****, P < 0.0001

Article Snippet: After 30 min of blocking with 5% non-fat milk, the membranes were incubated for the whole night at 4 °C with primary antibodies against ATF3 (1:500, ABclonal) and GAPDH (1:10,000, ABclonal).

Techniques: Single Cell, RNA Sequencing, Control, Expressing